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All images of tissue sections were captured using BZ-X710:BZ-X Viewer (Keyence? Total RNA was extracted from liver tissues with TRIzol reagent (Invitrogen, Carlsbad, CA, USA). The cDNA was synthesized from RNA with PrimeScriptTM RT Master Mix (Takara, Japan) for quantitative RT-PCR. The expression level normalized to Gapdh was analyzed by using the LightCycler 480 (Roche, Basel, Switzerland). The primer sequences are listed in Table 1.Tsurusaki Erotic Massage
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