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About Tokyo
Made me angry how people judge.
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But then, outta nowhere, it starts to rain. Like, seriously? I just got my takoyaki! I dash for cover under this little awning on Kōen-dōri. I’m standing there, all soggy and annoyed, when I see this group of kids playing in the rain. They’re laughing, splashing around, and I’m like, “Man, I wish I could be that carefree.”
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For mtDNA mutation analysis, we selectively amplified mtDNA from all 88 samples derived from 27 embryos using polymerase chain reaction (PCR) and performed mtDNA sequencing via next-generation sequencing. The success rate for data acquisition of each sample were 92.9% (52/56) for pre-culture samples, and 96.9% (31/32) for post-culture samples. Supplementary Table S1 lists mutations for each sample with a heteroplasmy level of ≥90%. The mean number of mtDNA mutations per embryo with a heteroplasmy level of more than 90% were 0.6 ± 0.8 (mean ± SD).Ijuin Find A Prostitute
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