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First off, I hit up the local café on Kōen-dōri. You know, the one with the cute barista? Yeah, her name’s Yuki. She always gives me that smile. Anyway, I ordered my usual—matcha latte, extra foam. But guess what? They ran outta matcha! Like, how do you run outta matcha in Japan? I was like, “Are you kidding me?”
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All images of tissue sections were captured using BZ-X710:BZ-X Viewer (Keyence, total RNA was extracted from liver tissues with TRIzol reagent (Invitrogen, Carlsbad, CA, USA). The cDNA was synthesized from RNA with PrimeScriptTM RT Master Mix (Takara, Japan) for quantitative RT-PCR. The expression level normalized to Gapdh was analyzed by using the LightCycler 480 (Roche, Basel, Switzerland). The primer sequences are listed in Table 1.Tsurusaki Sex Dating
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