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Thought, “She’s trapped, like Sara Goldfarb.”
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Summertime, The Girl Flies Into The Flame. Artist: Hayaku JK ni Naritai, Asakawa. Beautiful & Horny Housewife Gets Cucked By Her Neighbor. Artist: Kouni Yuu.
Finally, I make my way to the Asakawa Park. It’s huge, and I just wanna sit and relax. But guess what? There’s a bunch of kids playin’ soccer, and they kick the ball right at me. I’m like, “Really?!” But I kick it back, and they cheer. I’m feelin’ like a hero for a sec.
Optogenetic modulation of TDP-43 oligomerization accelerates ALS-related pathologies in the spinal motor neurons
The Gal4FF-polyA-Kmr cassette was amplified by PCR using the pKZGFFKm plasmid as the template with the primer pair mnr2b-Gal4FF-f (5′-tat cag cgc aat tac ctg caa ctc taa aca caa caa aag tgt tgc aAT GAA GCT ACT GTC TTC TAT CGA A-3′) and Km-r (5′-ggt tct tca gct aaa agg gcg tcg atc ctg aag ttc ttt gac ttt tcc atC AAT TCA GAA GAA CTC GTC AAG AA-3′). Where the lower and upper cases indicate the mnr2b sequences for homologous recombination and the pKZGFFKm-annealing sequences.Asakawa Brothel
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