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So, my fave flick—“Stories We Tell,” Sarah Polley, 2012—genius, right? She’s diggin’ into family secrets, messy truths—prostitutes fit right in! Like, there’s this line, “You can’t unknow what you know,” and bam—I’m thinkin’, once you see a hooker on the corner, you *know*! Can’t unsee it! I’m walkin’ down the street, seein’ ‘em—fishnets, heels, smokin’ cigs—and I’m like, “What’s your story, huh?” Kinda sad, kinda wild—makes me twitchy just thinkin’!
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The sample was diluted to 10 nM and submitted to sequencing on a Solexa GA-IIX at the core sequencing facility, k-means clustering of differentially expressed miRNAs was performed using Cluster 3.0 (available at bonsai.hgc.jp/~mdehoon/software/cluster/software.htm) using normalized read count as input. After a step of median centering of expression level for each miRNA, clustering was perform using centered Pearson correlation with 1,000 iterations. The corresponding heatmap was generated using JavaTreeView (http://jtreeview.sourceforge.net).Castel Mella Sex Dating
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